1. "MatLab program for precision calibration of optical tweezer", Computer Physics Communications 159 (2004) 225–240
Blocking, as opposed to windowing, can reduce noise by data compression to points with any desired distribution on the first axis. This is the reason we prefer blocking. Equidistant points on a linear and on a logarithmic axis are two particularly useful choices, the former for fitting to data, the latter for display of data in a log–log plot.Matlab program for calibrating optical force with power spectrum from experimental data.
This software is based on the following paper and includes all the details:
"Power spectrum analysis for optical tweezers", Rev. Sci. Instrum. 75 (2004) 594
2. TweezPal - Optical tweezers analysis and calibration software.
Calibration method:
Once the bead positions are obtained there are two methods to calibrate the trap. The more complex one is a calibration based on the power spectrum of fluctuations[1-3], where the bead radius and the viscosity of the liquid have to be known. A simpler calibration method based on the position fluctuations using a statistical approach [4], this method does not rely on specific information about the medium or the trapped particle. It is independent of viscous drag, particle size and shape, and needs only the absolute sample temperature T as a parameter.
[1] "Power spectrum analysis for optical tweezers", Rev. Sci. Instrum. 75 (2004) 594.
[2] "MatLab program for precision calibration of optical tweezers", Comput. Phys. Comm. 159 (2004) 225–240;
[3] Tweezercalib 2.1: Faster version of MatLab package for precise calibration of optical tweezers, Comput. Phys. Comm. 175 (2006) 572–573.
[4] "Photonic force microscope calibration by thermal noise analysis", Appl. Phys. A 66, S75–S78 (1998)
Blocking, as opposed to windowing, can reduce noise by data compression to points with any desired distribution on the first axis. This is the reason we prefer blocking. Equidistant points on a linear and on a logarithmic axis are two particularly useful choices, the former for fitting to data, the latter for display of data in a log–log plot.Matlab program for calibrating optical force with power spectrum from experimental data.
This software is based on the following paper and includes all the details:
"Power spectrum analysis for optical tweezers", Rev. Sci. Instrum. 75 (2004) 594
2. TweezPal - Optical tweezers analysis and calibration software.
Calibration method:
Once the bead positions are obtained there are two methods to calibrate the trap. The more complex one is a calibration based on the power spectrum of fluctuations[1-3], where the bead radius and the viscosity of the liquid have to be known. A simpler calibration method based on the position fluctuations using a statistical approach [4], this method does not rely on specific information about the medium or the trapped particle. It is independent of viscous drag, particle size and shape, and needs only the absolute sample temperature T as a parameter.
[1] "Power spectrum analysis for optical tweezers", Rev. Sci. Instrum. 75 (2004) 594.
[2] "MatLab program for precision calibration of optical tweezers", Comput. Phys. Comm. 159 (2004) 225–240;
[3] Tweezercalib 2.1: Faster version of MatLab package for precise calibration of optical tweezers, Comput. Phys. Comm. 175 (2006) 572–573.
[4] "Photonic force microscope calibration by thermal noise analysis", Appl. Phys. A 66, S75–S78 (1998)